鸽源新城疫病毒全基因组序列分析及致病性研究

doi:10.11843/j.issn.0366-6964.2014.02.013

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (2): 255-261.doi: 10.11843/j.issn.0366-6964.2014.02.013

鸽源新城疫病毒全基因组序列分析及致病性研究

胡北侠¹，杨少华¹，张会娟²，伊惠²，张伟²，王莉莉³，许传田¹，张琳¹，黄庆华¹，张秀美^1*

（1.山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室，济南 250100；2.山东农业大学动物科技学院，泰安 271018；3.山东省微山县畜牧局，微山 277600）

收稿日期:2013-08-22 出版日期:2014-02-23 发布日期:2014-02-23
通讯作者: 张秀美（1956-），女，江苏徐州人，研究员，主要从事禽病综合防控技术研究，E-mail：zxm820410@163.com
作者简介:胡北侠（1974-），女，江苏东海人，硕士，副研究员，主要从事禽病综合防控技术研究，Tel:0531-88970857，E-mail：hubeixia917@sina.com
基金资助:
公益性行业（农业）科研专项（201003012；201303033）；现代农业产业技术体系建设专项资金（CARS-42-Z12）

Complete Genome Sequence Analysis and Pathogenicity of Newcastle Disease Virus Isolated from Pigeons

HU Bei-xia¹，YANG Shao-hua¹，ZHANG Hui-juan²，YI Hui²，ZHANG Wei²，WANG Li-li³，XU Chuan-tian¹，ZHANG Lin¹，HUANG Qing-hua¹，ZHANG Xiu-mei^1*

(1.Shandong Key Lab of Animal Disease Control and Breeding，Institute of Animal Science and Veterinary Medicine，Shandong Academy of Agricultural Sciences， Jinan 250100， China;2.College of Animal Science，Shandong Agricultural University，Tai’an 271018， China;3.Weishan Bureau of Animal Science and Veterinary，Shandong Province，Weishan 277600， China)

Received:2013-08-22 Online:2014-02-23 Published:2014-02-23

摘要/Abstract

摘要：

为了科学理解新城疫病毒（NDV）的遗传进化规律，对鸽源NDV基因组和致病特征进行了鉴定分析。从发病鸽群中分离鉴定1株NDV，命名为Pigeon/China/SDLC/2011，并对其进行全基因组测序分析和致病性评价。测序结果显示Pigeon/China/SDLC/2011基因组为15 192 bp，具有NDV强毒株 F蛋白裂解位点¹¹²RRQKRF¹¹⁷。遗传进化分析发现Pigeon/China/SDLC/2011属于基因Ⅵ型，其6个结构蛋白氨基酸序列与基因Ⅶ型NDV参考株相比存在不同程度变异，其中P和HN蛋白相似性最低，分别为84.6%~84.8%和90.0%~91.1%。致病性试验结果显示鸽感染后表现瘫痪、翅膀下垂或斜颈等神经症状和排绿色稀粪，病死率为70%~80%；而SPF鸡感染后没有明显的临床症状。试验鸽喉头和泄殖腔病毒检出率明显高于试验鸡。本研究结果显示Pigeon/China/SDLC/2011在分子特性和对SPF鸡致病性方面与基因Ⅶ型NDV存在明显差异。虽然SPF鸡感染Pigeon/China/SDLC/2011后没有发病死亡，但可向体外排毒，因此必须采取有效措施防止鸽源NDV在鸽群和鸡群之间的相互传播。

Abstract:

The genome and pathogenicity of pigeon NDV isolate were characterized for better understanding the evolution of NDV.A strain of Newcastle disease virus，named Pigeon/China/SDLC/2011，was isolated and identified from diseased pigeon flock.The full-length genome of Pigeon/China/SDLC/2011 was sequenced and compared with reference strains.Besides，the pathogenicity of Pigeon/China/SDLC/2011 was evaluated in pigeons and SPF chickens.Sequence analysis showed that the complete genome of Pigeon/China/SDLC/2011 contained 15 192 nucleotides(nt).The cleavage site of the fusion protein was¹¹²RRQKRF¹¹⁷，a feature generally associated with virulent NDV strains.Phylogenetic analysis，based on the genomic sequences and sequences of the fusion gene，revealed that Pigeon/China/SDLC/2011 should be classified as genotype Ⅵ NDV.Mutations were observed in the six structural proteins of Pigeon/China/SDLC/2011 compared with reference NDV strains of genotype Ⅶ.More mutations were localized in the P and HN protein than the other proteins and the amino acid sequence identities of P and HN proteins between Pigeon/China/SDLC/2011 and genotype Ⅶ NDVs was 84.6%-84.8%，90.0%-91.1%，respectively.In the challenge test，70%-80% of pigeons inoculated with Pigeon/China/SDLC/2011 showed a series of nervous disorders：bilateral or unilateral locomotor disturbances of wings or legs，torticollis，and watery green diarrhea and died in 6-14 days.However，chickens remained clinically normal throughout the study.Viruses were detected more frequently in the swabs of pigeons than chickens.The results showed that marked differences in molecular characterization and pathogenicity in chickens were recorded between Pigeon/China/SDLC/2011 and the virulent NDVs of genotype Ⅶ.Although Pigeon/China/SDLC/2011 was nopathogenic for chickens，some measures should be taken to prevent virus transmission between pigeions and chickens as viruses were detected in swabs of infected chickens.

中图分类号:

胡北侠，杨少华，张会娟，伊惠，张伟，王莉莉，许传田，张琳，黄庆华，张秀美. 鸽源新城疫病毒全基因组序列分析及致病性研究[J]. 畜牧兽医学报, 2014, 45(2): 255-261.

HU Bei-xia，YANG Shao-hua，ZHANG Hui-juan，YI Hui，ZHANG Wei，WANG Li-li，XU Chuan-tian，ZHANG Lin,HUANG Qing-hua，ZHANG Xiu-mei. Complete Genome Sequence Analysis and Pathogenicity of Newcastle Disease Virus Isolated from Pigeons[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 255-261.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/10.11843/j.issn.0366-6964.2014.02.013

https://www.xmsyxb.com/CN/Y2014/V45/I2/255

参考文献

［1］SAIF Y M.禽病学［M］．苏敬良，高福，索勋，主译．11版．北京：中国农业出版社，2005：108-130．
［2］KALETA E F，ALEXANDER D J，RUSSELL P H.The first isolation of the PMV-1 virus responsible for the current panzootic in pigeons［J］. Avian Pathol，1985，14(4)：553-557.
［3］ALEXANDER D J，RUSSELL P H，COLLINS M S.Paramyxovirus type 1 infections of racing pigeons.1.Characterization of isolated viruses［J］.Vet Rec，1984，114(18)：444-446.
［4］COLLINS M S，STRONG I，ALEXANDER D J.Pathogenicity and phylogenetic evaluation of the variant Newcastle disease viruses termed “pigeon PMV-1 viruses” based on the nucleotide sequence of the fusion protein gene［J］.Arch Virol，1996，141(34)：635-647.
［5］贾华敏.鸽I型副粘病毒JSG-4分离株生物学特性和免疫原性研究［D］.扬州：扬州大学，2006.
［6］刘秀梵，胡顺林.新城疫病毒的进化及其新疫苗研制［J］.中国兽药杂志，2010，44（1）：12-18.
［7］GUO H，LIU X，HAN Z，et al.Phylogenetic analysis and comparison of eight strains of pigeonparamyxovirus type 1 (PPMV-1) isolated in China between 2010 and 2012［J］.Arch Virol, 2013，158(6):1121-1131.
［8］ALEXANDER D J.Newcastle disease［C］// World Organisation for Animal Health.Manual of diagnostic tests and vaccines for terrestrial animals.6th ed.Paris，France，2008:576-589.
［9］杨少华，胡北侠，许传田，等.三株新城疫病毒强毒株的生物学特性及全基因组序列分析［J］.病毒学报，2012，28（2）：143-150.
［10］IORIO R M，SYDDALL R J，SHEEHAN J P，et al.Neutralization map of the HemagglutininNeuraminidase glycoprotein of Newcastle disease virus：domains recognized by monoclonal antibodies that prevent receptor recognition［J］.J Virol, 1991，65(9)：4999-5006.
［11］UJVÁRI D.Complete nucleotide sequence of IT-227/82，an avian paramyxovirus type-1 strain of pigeons (Columba livia) ［J］. Virus Genes，2006，32(1)：49-57.
［12］UJVÁRI D，WEHMANN E，KALETA E F，et al.Phylogenetic analysis reveals extensive evolution of avian paramyxovirus type 1 strains of pigeons (Columba livia) and suggests multiple species transmission［J］.Virus Res，2003，96(1-2)：63-73.
［13］ALEXANDER D J，PARSONS G，MARSHALL R.Avian paramyxovirus type 1 infections of racing pigeons：4 laboratory assessment of vaccination［J］.Vet Rec，1986，118(10)：262-266.
［14］ALEXANDER D J，PARSONS G.Avian paramyxovirus type 1 infections of racing pigeons：2 pathogenicity experiments in pigeons and chickens［J］.Vet Rec，1984，114(19)：466-469.
［15］DORTMANS J C，KOCH G，ROTTIER P J，et al.Virulence of Newcastle disease virus：what is known so far? ［J］.Vet Res，2011，42:122.
［16］KOMMERS G D，KING D J，SEAL B S，et al.Virulence of six heterogeneous-origin Newcastle disease virus isolates before and after sequential passages in domestic chickens［J］.Avian Pathol，2003，32(1)：81-93.
［17］DORTMANS J C，ROTTIER P J，KOCH G,et al.Passaging of a Newcastle disease virus pigeon variant in chickens results in selection of viruses with mutations in the polymerase complex enhancing virus replication and virulence［J］.J Gen Virol, 2011，92(Pt2)：336-345.
［18］IRVINE R M，ALDOUS E W，MANVELL R J，et al.Outbreak of Newcastle disease due to pigeon paramyxovirus type 1 in grey partridges (Perdix perdix) in Scotland in October 2006［J］.Vet Rec，2009，165(18)：531-535.
［19］ALEXAMDER D J，MORRIS H T，POLLITT W J，et al.Newcastle disease outbreaks in domestic fowl and turkeys in Great Britain during 1997［J］. Vet Rec，1998，143(8)：209-212.

[1]	段志强, 谢玲玲, 陈佳琪, 唐宏, 王燕碧, 赵采芹, 赵佳福. 新城疫病毒M蛋白与宿主蛋白相互作用的研究进展[J]. 畜牧兽医学报, 2022, 53(1): 32-42.
[2]	段志强, 谢玲玲, 周迪, 王燕碧, 赵采芹, 唐宏. 新城疫病毒M蛋白细胞核定位的机制与功能研究进展[J]. 畜牧兽医学报, 2021, 52(4): 891-898.
[3]	段志强，许厚强，嵇辛勤，陈强，胡炎，胡顺林，刘秀梵. 贵州省鸭源新城疫病毒强毒株的遗传变异分析及致病性研究[J]. 畜牧兽医学报, 2016, 47(8): 1623-1634.
[4]	崔鹏飞，彭志，张芳，关立峥，肖丽，李莉莎，邓国华，陈化兰. 四株鸭源H3N8亚型禽流感病毒对SPF鸡的感染性分析[J]. 畜牧兽医学报, 2016, 47(6): 1209-1214.

鸽源新城疫病毒全基因组序列分析及致病性研究

Complete Genome Sequence Analysis and Pathogenicity of Newcastle Disease Virus Isolated from Pigeons

RichHTML

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics

本文评价